Regulatory
PyeaR

Part:BBa_K3425130

Designed by: Emelie Bringeland, Therese Koivula   Group: iGEM20_UofUppsala   (2020-10-17)


YeaR Regulatory Promoter


The yeaR promoter is a part of the yeaR-yoaG operon which plays a role in nitrate and nitrite-responsive control in E. coli. PyeaR is repressed by the protein NsrR and, in the presence of said nitrates and nitrites, activated by the response regulator NarL. Since PyeaR is native to E. coli, it can be used alongside the two-component system NarX-NarL.


References

Lin, H.-Y., Bledsoe, P. J., and Stewart, V. (2007) Activation of yeaR-yoaG Operon Transcription by the Nitrate-Responsive Regulator NarL Is Independent of Oxygen- Responsive Regulator Fnr in Escherichia coli K-12. JB. 189, 7539–7548

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//promoter
Parameters
biologyE. coli
functionRegulatory promoter
negative_regulatorsNsrR
positive_regulatorsNarL